17, 18, 19 We observe that WRN is recruited at both RPA and γ-H2AX foci; however, Western blot and chromatin fractionation were performed as described.
Phosphorylated histone H2AX (γ-H2AX) is a potential regulator of DNA repair and is a (b) Representative data of western blotting for γ-H2AX in liver tissues.
View All Images (12) FC experiment of HepG2 using 10856-1-AP 1X10^6 HepG2 cells were stained with 0.2ug Histone H2A.X antibody (10856-1-AP, red) and control antibody (blue). While methods such as western blots and immunohistochemistry are widely used but difficult to validate to regulatory standards, the ELISA method is the most quantifiable and easiest to validate. To address this need Trevigen’s quantitative pharmacodynamic HT -H2AX assay measures -H2AX levels in cellular extracts Anti-phospho-Histone H2A.X (Ser139), clone 6L16 ZooMAb, is a recombinant Rabbit Monoclonal Antibody that specifically targets phospho Histone H2AX (ser139) and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), Peptide Inhibition Assay, and Western Blotting. Bethyl Laboratories Anti-Phospho-gamma-H2AX (Ser139) Polyclonal, Catalog # A300-081A. Tested in Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC) and Immunohistochemistry (IHC) applications. This antibody reacts with Human, Mouse samples. Supplied as 100 µL purified antibody (1 mg/mL).
For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween ® 20 at 4°C with gentle shaking, overnight. NOTE: Please refer to primary antibody product webpage for recommended antibody dilution. A. Solutions and Reagents Because phosphorylation of H2AX at Ser 139 (γ-H2AX) is abundant, fast, and correlates well with each DSB, it is the most sensitive marker that can be used to examine the DNA damage produced and the subsequent repair of the DNA lesion. γ-H2AX can be detected by immunoblotting and immunostaining using microscopic or flow cytometric detection. 2016-03-31 · Western blot - Anti-gamma H2A.X (phospho S139) antibody (ab2893) HeLa (Human epithelial cell line from cervix adenocarcinoma) cells were incubated at 37°C for 3h with vehicle control (0 µM) and different concentrations of camptothecin ( ab120115 ). 2010-02-04 · Gamma-H2AX in recognition and signaling of DNA double-strand breaks in the context of chromatin.
Reactivity Human, Mouse, Rat, Canine Host Rabbit Isotype IgG Vial size 0.1 ml Concentration 1.0 mg Western blot analysis of extracts from HeLa and HT-1080 cells, untreated or treated with UV, using Phospho-Mre11 (Ser676) Antibody (upper) or total Mre11 Antibody #4895 (lower). Western blot analysis of extracts from Jurkat and K562 cells, using RAD50 Antibody. 2003-02-01 Many toxic agents can cause DNA double strand breaks (DSBs), which are in most cases quickly repaired by the cellular machinery.
quantification. This method, as opposed to Western blot assay and Flow cytometry, provides more accurate HTB140 melanoma cells were exposed to γ -rays, in the dose range from 1 histone H2AX becomes phosphorylated ( Mischo et al.
Munira K. We. CELOD. för att mäta DNA-skador och reparation (analys av gammaH2AX foci, Ytterligare meriter inkluderar vana med Western blot-analys och immunoblotting techniques (Western Blot, Dotblot, SDS-PAGE, Bioanalyzer), Western Blot and different cell assays with both primary cells and cell lines.
Anti-phospho-Histone H2A.X (Ser139), clone 6L16 ZooMAb, is a recombinant Rabbit Monoclonal Antibody that specifically targets phospho Histone H2AX (ser139) and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), Peptide Inhibition Assay, and Western Blotting.
This antibody reacts with All, Human, Mouse, Rat samples. Supplied as 100 µg purified antibody (1 mg/mL). Bethyl Laboratories Anti-Phospho-gamma-H2AX (Ser139) Polyclonal, Catalog # A300-081A.
Invitrogen Anti-Phospho-gamma-H2AX (Ser139) Recombinant Monoclonal (RM224), Catalog # MA5-33062. Tested in Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (ELISA) and Luminex (LUM) applications. This …
Bethyl Laboratories Anti-Phospho-gamma-H2AX (Ser139) Polyclonal, Catalog # A300-081A. Tested in Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC) and Immunohistochemistry (IHC) applications. This antibody reacts with Human, Mouse samples.
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2010-02-04 · Gamma-H2AX in recognition and signaling of DNA double-strand breaks in the context of chromatin. Nucleic Acids Res 2008; 36 : 5678–5694. CAS PubMed PubMed Central Google Scholar Gamma H2AX (gammaH2AX) is the phosphorylated version of histone H2AX and is a marker for double-stranded breaks (DSBs) caused by DNA damage (1-4).
Compare Anti-gammaH2ax Antibody Products from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more. 2011-06-01
gamma-H2AX Antibody A300-081A-T TDetection of mouse gamma-H2AX by western blot.
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Erfarenhet av tekniker för att mäta DNA-skador och reparation (gammaH2AX foci, Ytterligare meriter inkluderar vana med realtids-PCR, Western blot-analys,
PA1-25001 has been successfully used in Immunoflourescence, and Western blot procedures. By Western Blot a band of ~15 kDa is detected. 3 reviews. Compare Anti-H2AX Antibody Products from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more. Western blot shows lysates of HEK293 human embryonic kidney cell line, K562 human chronic myelogenous leukemia cell line, Balb/3T3 mouse embryonic fibroblast cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat Histone H2AX Monoclonal Antibody (Catalog # MAB3406) followed by HRP H2AX is a member of the histone H2A family.